Coding
INT7

Part:BBa_K3254007:Design

Designed by: Yeqing Zong   Group: iGEM19_GENAS_China   (2019-10-10)


Integrase Int7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 153
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 814
    Illegal AgeI site found at 1215
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was first reported in 2014. Two PstI site were eleminated by synonymous mutations.


Source

The original organism is the Geobacillus sp. Y412MC61. The DNA was coloned from the source plasmid(pCis2+7+8+5, see reference).

References

Yang, L., et al., Permanent genetic memory with >1-byte capacity. Nat Methods, 2014. 11(12): p. 1261-6.